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Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for global nosocomial dominance.
S, sensitive; R, resistant. Published online Jul The Kpn and KpnJ strains showed resistance to all beta-lactams and fluoroquinolones. J Antimicrob Chemother National Center for Biotechnology InformationU.
However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid cjm both isolates. Author information Article notes Copyright and License information Disclaimer.
Frequency of BKCProducing Klebsiella Species Isolates
We verified that, in the absence of selective pressure, the plasmid harboring the bla BKC-1 gene, p, was unstable. Santosb Jorge L. The Kpn and KpnJ strains showed different plasmid profiles. A Map of Brazil, showing the geographic distribution of Klebsiella isolates that were investigated for the bla BKC-1 gene.
Antimicrob Agents Chemother Address correspondence to Willames M. Since BKC-1 is a weak carbapenemase and other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype.
Plasmid extraction of K. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. BKC-1 is a new class A serine carbapenemase that was recently identified in Jdm pneumoniae clinical isolates. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard— 10th ed CLSI document MA To evaluate the stability of p harbored by the Kpn isolate, we performed serial passages of the Jc isolate on Mueller-Hinton agar plates without the addition of antibiotic.
Open in a separate window. The two BKCproducing isolates belonged to clonal complex and possessed identical pulsed-field gel electrophoresis patterns. Journal List Antimicrob Agents Chemother v. Martinsa Adriana G. Support Center Support Center. Antimicrobial susceptibility profiles of BKCproducing Jvm. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only effective tools for preventing the acquisition and spread of new carbapenemase-encoding genes.
Результаты поиска для JCM-5046
This article has been cited by other articles in Ncm. For comparison purposes, the first reported BKC-producing strains, i. Among the Klebsiella strains, The other authors have no conflicts to declare.
Transformant cells were selected on LB agar plates supplemented with 0. These carbapenem resistance genes are frequently harbored on mobile genetic elements that are highly transmissible, contributing to the increasing frequency of carbapenem-resistant Enterobacteriaceae 1.
NBRC Strain Passport – StrainInfo
Frequency of BKCproducing Klebsiella species isolates. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates.
To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ. Kpn showed susceptibility to tigecycline MIC, 0. BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that was recently identified in 55046 pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2. These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications.
The Klebsiella strains were isolated from different clinical specimens obtained between and Fig.